Title: Junior ResearcherPosition Number: 0084128THiring Unit: UH, School of Medicine, Department of Anatomy, Biochemistry and Physiology - IBRLocation: UH ManoaDate Posted: October 05, 2018 (If approved today.)Closing Date: Continuous - application review begins October 23, 2018Salary Information: Commensurate with education and experienceMonthly Type: 11 MonthTenure Track: Non TenureFull Time/Part Time: Full TimeTemporary/Permanent: Temporary - Not to Exceed: March 31, 2019 Other Conditions: To begin immediately, subject to position clearance; renewal dependent upon performance and availability of funds. Duties and Responsibilities
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As part of a research team, conduct research investigating the role of sex chromosome genes in gamete formation.
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Perform various techniques necessary for in vitro reconstitution of gametes and their molecular and functional assessment, some of which include stem cell isolation, culture and genetic modification, cytogenetic and molecular biology analyses of various cells, and assisted reproduction technologies.
Assist in developing specific cellular and molecular techniques as needed by the project.
Assist the PI with data analysis and interpretation, preparation of manuscripts, and data presentations.
Supervise undergraduate and graduate students as assigned.
Other scholarly duties as assigned.
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Master's degree from an accredited four-year college or university in the field of medical, biological or animal science.
Demonstrated knowledge and training in molecular biology, reproductive biology and developmental biology, and cell biology.
Ability to perform the following: cell isolation and culture, PCR, RT-PCR, qRT-PCR, gel electrophoresis, western blotting, immunohistochemistry.
Demonstrated ability to perform research as part of a working team.
Poise and good address for meeting and conferring with others.
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PhD degree from an accredited four-year college or university in the field of medical, biological or animal science.
Knowledge and experience with mouse genetics, stem cell culture, germ cell manipulations, micromanipulation of gametes and embryos.
Ability to perform vector preparation, lentiviral transduction, sequence analysis, and FACS.
Knowledge and documented experience with analyses of mouse spermatogenesis and oogenesis and in vitro fertilization.
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Strong experience in the ESC and iPSC line establishment, culture, genetic manipulations and reprogramming, as well as working knowledge of assisted reproduction technologies.
A record of publications in peer reviewed scientific journals.
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